The Trumps and Morning Joe hosts once had a very cozy relationship with Donald giving the MSNBC pair-who became romantically involved and recently got engaged-interviews when he was running for President.
Meanwhile, White House social media director Dan Scavino tore into Brzezinski and Scarborough on Twitter by saying the "Morning Joe" co-hosts are "lost, confused and saddened" because President Trump won't return their calls.
"It's been fascinating and frightening and really sad for our country", she said on the programme. "Also, those records show I haven't spoken with you in many months", the host wrote.
"The fake media is trying to silence us, but we will not let them", Trump said, without offering any evidence that journalists - who cover every public remark made by the President - have attempted to silence him.
He called Mr Scarborough "Psycho Joe".
Scarborough said the president has a "disturbing obsession with Mika".
"What does that say to our allies?" What is that saying to our enemies?
Brzezinski and Scarborough aren't the only ones who've had pointed responses to Trump's tweets.
Mrs Trump denied having a relationship with the U.S. presenter. I say the same thing [to them that] I say on the show: I think he's got a real problem with women, and that this presidency is a setback.
The hosts said they have noticed a change in his behaviour over the past few years which left them neither shocked nor insulted by the Thursday tweet.
Trump also escalated his war with media at an event to honour USA military veterans ahead of July 4, the American Independence Day. Mr Trump tweeted during Friday morning's broadcast, "Watched low rated @Morning_Joe for first time in long time".
Those White House calls had been preceded by Enquirer reporters calling Brzezinski's teenage children and close friends, she said.
Mr Trump's tweets, his latest attacks on the United States news media, were roundly condemned by lawmakers in both parties and became a distraction as his fellow Republicans in the Senate try to iron out their differences over major healthcare legislation.
"Obviously I don't see that as an appropriate comment", said Republican House Speaker Paul Ryan.