In 2016, McGowan tweeted that she had raped by a Hollywood executive and on Thursday afternoon, in a series of tweets directed at Amazon CEO Jeff Bezos, the actress alleged that Harvey Weinstein raped her.
A number of high-profile women were among those calling for a boycott, with several highlighting examples of abuse they had suffered online which had not resulted in the suspension of perpetrators' accounts.
Twitter said Ms McGowan's account had been temporarily locked because one of her tweets violated its terms of service.
Rose is one of a host of stars who allege the disgraced movie mogul sexually harassed or assaulted them, and had been using Twitter to denounce those she viewed as complicit in protecting him. Her account has now been restored, and she is back, fiercer than ever.
Chrissy Teigen said, "Ladies".
Many men have also lent their support, including actors Mark Ruffalo and Terry Crews.
The boycott is meant to last 24 hours, and while a large number of women have declined to participate, it certainly appears as though the platform will be a little quieter today.
Robin Williams' death in 2015 led some Twitter users to send vicious messages to his daughter, prompting her to delete the app from her phone.
In its response to McGowan on Thursday, Twitter said it would be "clearer about these policies and decisions in the future". "Twitter is proud to empower and support the voices on our platform, especially those that speak truth to power".
Twitter didn't respond to a request for comment.
"Some visible users have left the platform, but it hasn't seemed to make Twitter sit up and take notice".
The idea for the boycott was suggested in a tweet by engineer Kelly Ellis as a way to show solidarity for actress Rose McGowan and "all the victims of hate and harassment Twitter fails to support".