Supporters of the deal fear Trump's decision to decertify the deal could eventually unravel it, causing more tension in the crisis-hit Middle East, where Shi'ite Iran is involved in a decades long proxy war with US ally Sunni Saudi Arabia.
In a speech on state TV, he said Tehran would stick to the agreement, but only as long as the other six signatories - the US, UK, Russia, France, China, and Germany - respected the deal.
"But we will try on our side to obtain the comfort that we can continue the project, which will be our priority", he added. "I don't think there is a majority in the U.S. Senate that wants to abolish the deal".
"They are angry as today the Islamic republic of Iran has disrupted their plans in Lebanon, Syria and Iraq", Khamenei said. We welcomed this, but it is not enough to ask Trump not to rip up the agreement.
European Union Foreign Policy Chief Federica Mogherini, too, said the 2015 nuclear deal is "working", stressing that the USA can not unilaterally cancel the global agreement.
Under the deal, Iran agreed to curb its disputed uranium enrichment program in return for relief from global sanctions that crippled its economy, and United Nations nuclear inspectors have repeatedly certified Tehran's compliance with the terms. Pouyanne has said Total would withdraw from Iran if obliged by law.
"They must avoid interfering in our defence programme", Khamenei said. "We will not accept the Europeans going along with America's bullying". Why do you have missiles yourselves?
The senior commander went on to say that not only European countries and the International Monetary Fund (IMF) refused to comply with Trump's stance toward Iran, but even his own defense and state secretaries held him in contempt for such a stance.
It has called upon the Islamic Republic not to "undertake any activity related to ballistic missiles created to be capable of delivering nuclear weapons".
"To ask the same thing (as the Americans) about why Iran is present in the region - well, deal with it".