Blake struggled for balance after a messy start and finished with the bronze in a time of 10.19 seconds, after qualifying fastest with a time of 10.06 in the semi-finals.
Simbine's win was least expected as pre-event favourite Yohan Blake had boasted of there "being plenty in the tank" after last night's semifinals.
"It's my first worldwide title and for me it's a milestone, a stepping stone towards the world championships, and the Olympics and more competitions and more global competitions", said the 24-year-old.
"I was stumbling all the way and I didn't recover from it", said the 28-year-old. "I felt lacking in confidence, like silver wasn't good enough but I know I did the best race I could". I knew if I managed to do so, I would get a good result.
Blake's compatriot and athletics great Usain Bolt congratulated his former team mate and Simbine on Twitter.
"Keep putting in the work".
Before the start of the Games, Simbine said he would no longer merely hope for the best going into a major championship but go out with the mindset of winning gold. Simbine's victory ended Jamaica's domination of the Commonwealth 100m dating back to the 2006 Games in Melbourne.
"After the 100m yesterday I felt happy with it but I felt media-wise it was a bigger deal that I didn't get gold, that it wasn't an achievement to win silver", she said.
"I wasn't focused on (Blake)".
"Being able to run in lane eight and lane nine with Henricho and placing first and second with him, it's really wonderful".
Michelle-Lee Ahye held off a two-pronged Jamaican challenge to claim the women's 100m gold for Trinidad and Tobago in 11.14. What more it was South Africa's first ever gold medal over the distance.
The South Africa flag bearer raced within herself but still qualified fastest for Tuesday's final ahead of home runner Georgia Griffith (4:06.41) and Kenya's Mary Kuria.
But there was delight for Andy Pozzi after the new world indoor 60m hurdles champion qualified fastest for today's 110m hurdles final.
After crossing the line, the 31-year-old did two push-ups on the track to show his fitness and grinned at the TV camera.
Meanwhile, Wales' Hollie Arnold added Commonwealth gold to her World and Paralympic titles in the F46 javelin in dramatic style, producing a world-record final throw to snatch victory from New Zealand's Holly Robinson.
Samoa had also only won three Commonwealth gold medals before Mao picked up their first for these Games with a total lift of 360kg to beat Indian favourite Pardeep Singh in the men's 105kg class.