Simbine was able to capitalise with a solid run, taking gold in an underwhelming winning time of 10.03 seconds.
Bruintjies produced the best performance of his career finishing ahead of former world champion Yohan Blake winning the silver medal in 10.17.
"I'm doing some good work in training and my confidence is coming back", said Blake, who has a personal best of 9.69.
"This is probably my best achievement so far.it's my first worldwide title and for me it's a great feeling".
He has featured in the 2016 Olympic and the 2017 World Championships finals only two five-placed finishes to show for it.
In the pool, Cate Campbell won the 50m butterfly to lead Australia to a three-gold haul, but there was a major shock when Scotland's Duncan Scott produced a thunderous finish to upset a heavyweight field in the 100m freestyle.
Jamaican star Blake was the red-hot favourite heading into the first Commonwealth Games 100m event without the retired Usain Bolt.
Channel 7 commentator and Aussie athletics great Tamsyn Manou said Blake stiffened after getting a poor start out of the blocks - a relatively novice error. "I was just focused on myself and making sure I get to the 50 meters as quick as I can because I know if I put that together the rest of my race will come together", said the Pretoria 24-year-old.
A stumbling Blake struggled for balance and by the time he regained it, the race was over, leaving him disappointed with the bronze and a time of 10.19.
"Usain's good... he wants me to keep it basic, don't think too much, just hold your head and get the job done", added Blake.
'I was stumbling all the way.
The South Africans topped the podium.
The third fastest man of all time, Blake topped qualifying with a time of 10.06 and had been warned jokingly by Bolt not to come home without gold in his luggage. "Usain told me 'Yohan, you've got to win, you know what it means, you know you can't go back home'".
"It is an incredible feeling to come here and do what I could have done, and I've won the gold medal which is something I knew I could do".
Trinidad and Tobago's Michelle-Lee Ahye triumphed in the women's race, her first gold medal at a major global meet.
Steven Kari slammed the floor in delight and hurled his belt into the crowd as he won the men's 94kg weightlifting to earn Papua New Guinea their first gold of the Games.
It was the first time in 20 years the 100m crown was not retained by a Caribbean nation.
"It's my first global title and for me it's a milestone, a stepping stone towards the world championships, and the Olympics and more competitions and more worldwide competitions", said Simbine.
"I just stayed calm and did what I had to do".
Stella Chesang fought off Kenya's Stacy Ndiwa to take the 10,000m gold for Uganda and win her maiden Commonwealth medal.
New Zealand's world champion Tomas Walsh threw 21.41 meters to take the shot put gold, going one better than his silver at Glasgow.