Khamenei reiterated that he knew that "accepting the nuclear deal would not end America's enmity". "And the Islamic Republic will still be standing".
"I said many times from the first day: Don't trust America", Khamenei said.
In a declaration on Tuesday, President Donald Trump officially withdrew the United States from the nuclear pact.
The first official to react was President Hassan Rouhani who went on live TV nearly immediately after Trump's statement on Tuesday. The same behavior existed in previous USA presidents.
Trump abandons Iran nuclear deal: What now?
According to the website of Iran's Parliament "Khaneh Mellat", the MPs state that Iran nuclear deal with the world was aimed at lifting sanctions and opening in Iran's economy, neither of which has been fulfilled despite Iran fully compliance with the deal.
Raising the issue of Iran's nuclear program was an excuse to curb the Islamic Republic's regional influence and missile program, Khamenei said.
He described Iran as the most developed states in technical, scientific and academic fields in the region and expressed the hope that his country could benefit from Iran's achievements.
On the media front, reformist and other pro-Rouhani newspapers praised Rouhani's decision to remain in the JCPOA with European partners, Russian Federation and China, but hardline daily Kayhan, close to Khamenei, borrowed language from previous Trump and Khamenei speeches, and opined that now that the U.S. has shredded the JCPOA, it is time to set the deal on fire.
The deal with Iran's government was signed by Mr Trump's predecessor, Barack Obama.