Rebels seized control of the crossing in 2015, cutting a major lifeline for Syrian exports and disrupting a major trade route between Syria, Jordan, Lebanon and oil-rich gulf counties.
Rebels in Syria's battered south are returning to talks with government ally Russian Federation, an opposition spokesman said on Thursday, after almost a full day of unprecedented bombing on their positions.
In recent weeks, in defiance of a deescalation agreement, Syrian and allied forces have stepped up attacks on the areas in southwestern Syria bordering Jordan and Golan Heights in an effort to dislodge the rebels from that area.
Concrete blocks near the Nassib border crossing were plastered with emblems of insurgent factions, while an army checkpoint at the entrance carried photos of Assad.
As you can imagine, more people are reading The Jerusalem Post than ever before. The Syrian flag flew in the distance, along with what appeared to be a Russian flag.
Friday's reported takeover comes after rebels announced reaching a deal with Russian Federation, an ally of the Syrian government, aimed at ending a devastating government campaign in the province of Daraa in southern Syria.
"An agreement has been reached between the Syrian government and the terrorist groups", which includes "the handover of heavy and medium weapons in all cities and towns", Syria's official news agency SANA reported.
According to the United Nations, more than 320,000 people have fled the recent fighting in Dara'a since June 19.
The UN-monitored 1974 armistice bars or limits military build-ups by either side around the Golan.Israel warned on Thursday it would attack Syrian regime forces if they deploy in a demilitarised zone while advancing against opposition rebels in the south, including Daraa and Quneitra.
An Associated Press journalist escorted by the Syrian army on Saturday saw troops setting up checkpoints along the largely deserted road leading up to the crossing, full of potholes from shelling; they removed sand barriers and burnt out cars by its side.
Both Israel and Jordan, which beefed up their border controls, said they would not let refugees in but distributed aid inside Syria. "We should have been cautious by our friends and the betrayal of friends", the spokesman for the rebels' joint operations room, Ibrahim Jabawi, said bitterly.
One officer told the TV outlet that troops have taken up positions along the border with Jordan and are removing illegal crossing points.
Waves of air strikes pounded rebel-held areas of southern Syria after the failure a day earlier of Russian-brokered talks to end the offensive in Daraa province, which has killed dozens and forced tens of thousands from their homes.