Almost half of respondents - who work in retail, food and manufacturing - say production costs have climbed, and 42 percent said they've noticed a decreased demand for their products.
U.S. President Donald Trump last week said that in addition to preparing tariffs on another $200 billion worth of goods, he had tariffs on another $267 billion worth of goods "ready to on short notice if I want".
The two governments have imposed 25 percent tariffs on $50 billion of each other's goods.
Stocks in Europe rose earlier as prospects for U.S.
The European Union Chamber of Commerce in China released its own survey on Thursday saying the tariffs were causing "significant disruptions" to global supply chains and "seriously impacting" non-Chinese and non-American companies.
China welcomed on Thursday a U.S. offer to hold fresh trade talks, adding that the two are discussing the details and providing some hope the world's top economies could step back from the brink of an all-out trade war.
"With the $200 billion round of tariffs looming, this is probably the best time for the U.S. to go back to the table with a "take it or leave it" type of trade demand", Robert Carnell, the chief Asia-Pacific economist at investment bank ING, said in a note.
The timing and location of the proposed meeting were unclear, the sources familiar with the matter said.
The White House is under enormous pressure from U.S. companies to resolve its differences with China swiftly before either country imposes more tariffs or restrictions that these firms argue could hurt growth and lead to lost American jobs.
"The U.S. administration runs the risk of a downward spiral of attack and counterattack, benefiting no one", Zarit said in statement.
Robert Holleyman, former deputy U.S. trade representative, said USA demands for fundamental changes in China's state-directed economy would make for hard talks. -China trade relations, address long-standing inequities and level the playing field.
The Twitter post came after White House officials confirmed that Treasury Secretary Steven Mnuchin had offered a formal invitation to Chinese leaders to restart trade talks, aimed at de-escalating the trade battle between the world's largest economies. They said almost one-third are thinking about canceling or postponing investment decisions.
Beijing has pledged to hit back with five to 25 per cent tariffs on $60 billion in U.S. imports in retaliation for any measures from the US.
Some 52.1 percent of companies said Chinese authorities are slowing customs clearances, increasing inspections or imposing other "qualitative measures".
"I think most of us think it's better to talk than not to talk, and I think the Chinese government is willing to talk", he said.
The U.S. -China Business Council (USCBC), a nonpartisan non-profit representing around 200 American companies, told Observer that, in recent weeks, the council has been hearing from U.S. companies about delays and challenges getting licensing approvals in China. The Chinese government has dismissed the complaints as "groundless".