The ICC has suspended the Windies skipper for the upcoming game.
Kraigg Brathwaite soldiered through the session in getting to 48 not out, anchoring the home side's effort to bat through the day in pursuit of a significant lead as they press for the victory which will give them an unassailable 2-0 lead in the series and the Wisden Trophy back in their possession after ten years in English hands.
As the West Indies' second innings began, Joseph was preparing to bat as scheduled and was also expected to the field and bowl.
Holder was punished by the International Cricket Council for the team's slow over rate in winning the second test in Antigua on Saturday.
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John Campbell finishes the game with a six!
England bowled the hosts out for 306 in Antigua, taking their four remaining wickets for 34 runs on the third morning.
The all-rounder savoured the moment after so many hard days for the Windies. After an opening partnership of 35 runs, Rory Burns was the first man to depart for 16 runs. 14 runs later, Holder cleaned up Jonny Bairstow to put Windies well in command.
Holder, who led his side brilliantly once again, was full of praise for Joseph, who was given a great support from the crowd and applause from the England players so soon after receiving such devastating news. England kept on losing wickets at regular intervals and were eventually all out for 132.
Root said that England will try and assuage the stinging series loss by putting in a better show in St Lucia, the venue for the third and final Test of the series.
Tottering on 96 for six, England's decline continued when Ben Foakes was lbw to Roach for 13 on review and three balls later in the next over, Jos Buttler fell lbw to Holder, after top-scoring with 24 off 48 balls with five fours.
Opener Kraigg Brathwaite is expected to captain West Indies in Holder's absence, having led the side in two Tests against Bangladesh past year.