It was her third straight win in the Queen Elizabeth - the jewel in the crown of the annual Championships at Sydney Racing HQ.
And she showed her appreciation for Bowman by head-butting him and splitting his lip as he got off for the last time after she beat Japanese visitor Kluger and old adversaries Hartnell and Happy Clapper.
Legendary Australian sports broadcaster Bruce McAvaney said ahead of the race that her farewell sat comfortably alongside any of the great Australian sporting moments he had called or witnessed in his long career.
"I don't know what to say". Kluger looked to challenge as the finish line loomed in sight, but just could not rein in the incredible mare, who finished one-and-a-half lengths in front. "It has been very real for me all day, with the amount of people here, it is just wonderful", he told the Seven Network.
"It's over", Bowman said.
"Everyone watching around the world". America, Europe, Africa, all over Australia.
"So even though she is the best horse I have ridden, when people ask me who is the best horse I have ridden I automatically think Dundeel, who won this race too".
"There's not much more to say", he said.
Forty minutes before the Queen Elizabeth, the Waller-trained Verry Elleegant won the Australian Oaks to take the trainer's Group One tally to 99 and set the scene for a magic century.
But as the horses strolled around the final bend it was clear there was going to be no other ending than Winx saluting. My team do an fantastic job.
"But she should get away with it in the Oaks because she might just be better than them and away from the top three or four the class of the race falls away a bit".
Australian racehorse Winx retired on Saturday after winning her final race in Sydney, a record 33rd in a row. Well, I just do what I do.
"She needs to be remembered for winning today, if she didn't she will be remembered to be one of the greatest".
The streak that started on the Sunshine Coast in 2015 has lasted almost four years and almost always executed with a peerless burst of acceleration and an apparent human-like obsession with winning.