In Motihari and Samastipur one case each of AES have been reported.
The death toll due to AES has been on a steady rise for the past 10 days, with children being the worst hit.
"We are trying our best to save them", said S.P. Singh, the chief medical officer of Sri Krishna Medical College and Hospital.
According to the Hindustan Times, health authorities did not attribute the childrens' deaths to AES, but rather to hypoglycemia (low blood sugar).
A five-member team from the central government visited Muzaffarpur on Wednesday to investigate the cause of death of those children.
Pandey said creating awareness among the people is needed to prevent the outbreak of the disease. "I am sure the situation will be contained soon", Union Health Minister Harsh Vardhan told ANI.
Dozens of children have died in the northern Indian state of Bihar this month from a deadly brain disease believed to be linked to a toxic substance found in lychee fruit.
In the last two years, the number of deaths was around two or four, Rai said, adding the Centre and the state governments have taken requisite preventive measures and so far 69 children, affected with the disease, have been discharged from the two hospitals. It is characterized by high fever and inflammation of the brain.
Meanwhile, sick children with symptoms of AES continue to be admitted into the two crammed Pediatric Intensive Care Units (PICU) at SKMCH.
The Union Minister said a team from Norway has arrived to ascertain the reasons behind the deaths of children while samples were sent to a laboratory in Pune. The disease claimed a record 150 lives in 2014.Most children dying of the outbreak of AES belong to economically weaker sections of the society.
Encephalitis is a viral infection which causes mild flu-like symptoms such as high fever, convulsions, and headaches.