Biles won the vault by more than half a point, averaging 15.399 for her two attempts. Biles' U.S. teammate Jade Carey took the silver on 14.883, with Ellie Downie of Britain third on 14.816.
"I never think of medal counts or anything", she said after winning her record-tying 23rd and record-extending 17th gold in the vault final on Saturday, the penultimate day of worlds.
Later Saturday, Biles will have a chance to eclipse Scherbo's long-standing mark when she contests the uneven bars.
The 22-year-old plans to celebrate when she gets back home to Texas, where "my mom usually likes to throw a worlds party - whether I want one or not", she joked.
Less than a year before the Tokyo Olympics, she can potentially leave Stuttgart with five medals with the women's beam and floor finals to come on Sunday. "But it's getting up there and I just feel really proud".
Earlier in the week, Biles broke the record for most individual medals overall by a woman at the world championships.
"I couldn't tell you how many I have if you didn't tell me already".
Britain's Olympic champion Max Whitlock won his third world title in pommel horse, scoring 15.5 with a near-flawless routine.
"I don't know; I feel like it's not me", Biles said after her win, per the Times. "I feel like I never think of records. It definitely didn't go as well as the training past year, but going into the gym we'll focus a lot more on that vault", she said.
Biles scored 15.066 on the beam after an nearly flawless routine, going for a simpler dismount than the double-double she performed earlier in the championships.
Last year's bronze medalist, Yulo performed the most hard routine of any of the eight finalists to score 15.3 and beat Israel's Artem Dolgopyat by one-tenth of a point.
Biles scored a whopping 15.066 points on the afternoon's most technically demanding routine to win gold. Blowing kisses to the crowd wasn't meant as a goodbye to the world championships, she said.
Said, whose father died in February, earned bronze and knocked Greek Olympic still rings champion Eleftherios Petrounias off the podium and out of an automatic Olympic spot.
Dalaloyan also took bronze on the horizontal bar, behind Brazilian gold medallist Arthur Mariano, for his fourth medal this week, completing the set after an all-around silver and team gold.