Since taking office Trump has repeatedly denigrated the European Union, once describing the bloc as a "foe" when asked which nations or leaders he considered to be adversaries of the US.
The leaders do not use Trump's name, but the US president took dozens of questions from journalists on Tuesday during impromptu news conferences at the start of his individual meetings with Macron, Trudeau and NATO Secretary-General Jens Stoltenberg.
The ad was released late Wednesday on Twitter and by mid-morning Thursday had already been viewed more than six million times.
Tusk contradicted some other European leaders, saying that it was still better for both the EU and the United Kingdom if Brexit did not happen.
"As the President has said, Joe Biden claims that foreign leaders have told him they want him to win the election".
In the ad, Biden claims that electing Trump to four more years would make it very hard to "recover America's standing in the world".
Trump has experienced tension with other leaders during this summit.
"I just watched - watched his team's jaws drop to the floor", Trudeau says in the recording, eyes wide in imitation.
According to the Washington Post, "The pointed ad marks the continued fallout after Trudeau, French President Emmanuel Macron, British Prime Minister Boris Johnson and other dignitaries were caught on camera Tuesday engaging in a brief exchange apparently about Trump that quickly spiraled into an global incident".
Meanwhile Deputy Prime Minister Chrystia Freeland, speaking on CTV on Thursday, was hesitant to say that relations had been damaged at the summit.
Ms Ward explained the leaders were using "safety in numbers" to protect themselves from the President and questioned whether they were "true leaders". The Biden marketing campaign advert appeared to maneuver up that chortle monitor so it performed proper after Trump's boast about his document. His constant complaint has been that some of NATO's 29 members are not spending two per cent of their Gross Domestic Product (GDP) on defence, as agreed in 2014 as an indirect response to Russian aggression in Crimea.
CNN reported Biden's video was posted 30 minutes after Trump landed back on USA soil, in keeping with Biden's policy of not criticising the president while he is overseas.
It is reported that arrived the day before from London trump shared with the diplomats on their impressions of the forum of North Atlantic Treaty Organisation and expressed thoughts about the future of the Alliance.
"I mean, all of life really is like middle school", added Fox News anchor Shannon Bream.