"So while they have now had two negative tests, they will have to get tested again to get two negative tests in a row that are in accordance with PCB's testing process and the protocols we have set up", PCB CEO Wasim Khan said in a statement.
Pakistan's tour to England will continue to go ahead as per schedule in July-August despite 10 players of the touring party tested positive for the novel coronavirus that continues to threaten the entire world.
If the third round of tests on these players are also "negative" they will be able to travel to England.
Pakistan all-rounder Mohammad Hafeez's coronavirus test from being first positive and then negative has sparked a lot of outrage.
The board has said it will announce results of all tests on Saturday (June 27).
20 players to travel to England on Sunday.
"I would request the PCB to properly look after these players because if you don't, incidents like Hafeez getting himself tested privately will happen", Inzamam said. Fakhar Zaman, Mohammad Hasnain, Mohammad Hafeez, Mohammad Rizwan, Shadab Khan and Wahab Riaz will now have to pass another test next week before they could join the team in England.
Of the original 29-man squad, 18 received the all-clear following Thursday's checks, as well as 11 members of the support staff. Following the Covid positive test results, the board called upon fast bowler Musa Khan and Pakistan U-19 captain and wicketkeeper Rohail Nazir.
Pakistan will now quarantine and train at Worcestershire's Blackfinch New Road for 14 days, before a planned move to Derbyshire's Incora County Ground on July 13 to begin internal warm-up matches. Zafar Gohar, meanwhile, will team up with the squad on their arrival in England.The left-arm spinner played a solitary one-dayer for Pakistan in 2015, though he will only be involved in pre-match preparations.
Meanwhile, bowling coach Waqar Younis is heading from Australia to England via Dubai on Saturday to join the Pakistan squad there, and will be appearing for Covid-19 test after arriving there.
Musa and Rohail have been included in the first group of travellers, which also includes: Players - Azhar Ali (captain), Babar Azam (vice-captain), Abid Ali, Asad Shafiq, Faheem Ashraf, Fawad Alam, Iftikhar Ahmad, Imad Wasim, Imam-ul-Haq, Khushdil Shah, Mohammad Abbas, Musa Khan, Naseem Shah, Rohail Nazir, Sarfaraz Ahmed, Shaheen Shah Afridi, Shan Masood, Sohail Khan, Usman Shinwari, and Yasir Shah.