Spanish player, Paula Badosa has tested positive for COVID-19.
"I have some bad news", she tweeted.
Now 72 players are in a "hard quarantine" in Melbourne and must self-isolate in their hotel rooms for 14 days following positive tests on three charter flights. There have been confirmed positive COVID-19 cases from charter flights originating in Doha, Qatar; Abu Dhabi, United Arab Emirates and Los Angeles.
"I want to thank everyone there for their efforts, I'm devastated not to be playing out in Australia". "But I'll try to recover as soon as possible listening to the doctors".
"That's an unfortunate effect for anyone who becomes a confirmed case - the isolation period starts from when that case is confirmed", the state's chief health officer Brett Sutton said.
Badosa had previously complained about being subjected to a stricter lockdown after being on a flight with someone who tested positive upon arrival in Melbourne.
Badosa said in a since-deleted tweet that she had not been expecting to have to isolate, even if there had been a positive result on her plane.
The Australian Open is scheduled to run from February 8 to 21 after it was postponed by three weeks to allow for the period of quarantine after players arrive in the country.
Murray had withdrawn from the Delray Beach Open in Florida this month, saying he wanted to "minimise the risks" of contracting the virus through worldwide travel ahead of the Australian Open.
According to 7News in Victoria, Australia, Djokovic asked for players to be relocated to houses with tennis courts, given improved food, granted access to be with coaches and have their number of days in quarantine lowered.
MELBOURNE, AUSTRALIA - A general view of Rod Laver Arena during day six of the 2016 Australian Open at Melbourne Park on in Melbourne, Australia.
His last Grand Slam final appearance came when he won Wimbledon in 2016.
Also in Australia, the leading umpire Carlos Bernardes is believed to have suffered a minor heart attack while in one of the quarantine hotels.
Local media reported that world No. 1 Ash Barty apologised after she was seen maskless at a departmental store in Melbourne despite a Victoria state requirement that masks be worn inside indoor shopping centres.