He also pointedly declined to criticise Ghosn's detention conditions, despite some worldwide concern about the repeated extension of the auto executive's pre-trial detention.
Hironaka, 73, has won several high profile cases, helping acquit senior lawmaker Ichiro Ozawa and senior bureaucrat Atsuko Muraki.
The move came on the same day that the former Nissan and Renault boss reshuffled his legal team in Japan, where he remains held in a Tokyo jail.
Motonari Otsuru, who has thus far represented Ghosn, gave no reason for stepping down, but was not available for comment.
"Renault has gathered sufficient evidence to understand and regret the methods used by Nissan and its lawyers to seek interviews with Renault employees through the Japanese public prosecutor's office", they said. "He will mount a more thorough and aggressive defense".
A brief statement said only that "today Otsuru and (Masato) Oshikubo submitted letters of resignation to the court as the defence lawyers for the case of Mr. Ghosn".
Ghosn released a short statement thanking Otsuru for his team's "tireless and diligent work", and called him a "very capable and intelligent man and lawyer".
The surprise shake-up came on the eve of an expected first meeting between the Tokyo District Court, prosecutors, and defence lawyers to discuss the outlines of Ghosn's eventual trial.
Hironaka's office said the attorney was out. "I look forward to defending myself vigorously, and this represents the beginning of the process of not only establishing my innocence but also shedding light on the circumstances that led to my unjust detention".
To date, Ghosn is still named a director of Renault despite resigning from the above-mentioned positions.
Ohtsuru seemed more accepting of Ghosn's prolonged detention since his arrest on November 19.
Global and European auto demand, except in the event of a no-deal Brexit, should be stable this year with growth in Russian Federation and Brazil, Renault said.
Jean-Dominique Senard, newly-appointed Chairman of Renault, poses with Thierry Bollore, newly-appointed Chief Executive Officer of Renault, after French carmaker Renault's board of directors meeting in Boulogne-Billancourt, near Paris, France, January 24, 2019.