Anyone who does not meet the requirements will be denied boarding.
The CDC noted the growing emergence around the world of variants of the coronavirus, which causes COVID-19, and evidence that the new variants are more easily spread.
"With the USA already in surge status, the testing requirement for air passengers will help slow the spread of the virus as we work to vaccinate the American public", the CDC said in a statement.
This decision reflects current infrastructure challenges in these countries, including limited COVID-19 testing capacity and heightened demand for tests.
Anyone flying into the United States from an worldwide destination will soon be required to show proof they've tested negative for COVID-19 before boarding their flight.
All air passengers with flight plans to the United States will soon be required to get a viral test within three days before their flight departs, and to provide written documentation of their lab results, or documentation of having recovered from Covid-19, the agency said in a statement.
The United States has joined a string of other countries requiring global arrivals to take a pre-flight COVID test as it attempts to slow the introduction of new variants.
There are more than 22.7 million coronavirus infections in the USA as of January 12, according to Johns Hopkins, and more than 379,000 deaths. Travel by foreigners to the USA and by Americans to worldwide destinations in December was down 76% compared to a year earlier, according to trade group Airlines for America.
All passengers arriving into Ireland will have to provide a negative Covid-19 test prior to departure from this Saturday (16 January).
On Christmas Eve, the CDC ordered negative coronavirus tests for all travelers arriving from the United Kingdom after the discovery of a new coronavirus variant.
The CDC said it had delayed the introduction of the requirement to allow passengers and airlines to adapt but CDC director Robert Redfield wanted it did not eliminate all risk.
Travellers arriving from Great Britain and South Africa will still be obliged to self-isolate for 14 days after their arrival in Ireland, even if they undergo a second PCR test after entering the country.
Travellers from green and orange list countries, as set out by the European Union traffic light system, will not have to restrict their movements upon arrival in Ireland, but they will be expected to adhere to local public health guidelines.
When contacted last night, Tourism Minister Dionisio D'Aguilar said while the new requirement will no doubt be an added concern for visitors, he "breathed a sigh of relief" that the United States would allow travellers to get a PCR or antigen test for entry, the latter of which is cheaper and has a quicker turnaround time.
But the new order may stop or reduce spread of other versions of the virus, like the one recently identified in South Africa.